human genome u133 plus 2.0 microarray gene expression profiles Search Results


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Gas5-derived small RNA (pi-sno75) specifically upregulates TRAIL expression in breast cancer cells. ( A ) The mRNA profiling <t>microarray</t> analysis. The gene expression scatterplot of the piRNAs mixture group versus that of the control group was shown. Significantly downregulated genes were shown in green, while upregulated genes in red. ( B ) MCF7 cells were transfected with piRNAs derived from GAS5 respectively for 48h, TRAIL mRNA levels were analysed by quantitative RT-PCR (qRT-PCR) and normalized to GAPDH levels. ( C ) MCF7 cells were transfected with pi-sno75 at different concentrations. The qRT-PCR was performed to analyse the effect of pi-sno75 on TRAIL expression. ( D ) MCF7 cells were transfected with pi-sno75 and piNC for 48h, western blot was performed to analyse TRAIL protein expression. ( E ) In MCF7 cells transfected with pi-sno75, the mRNA levels of TRAIL and other genes were determined by qRT-PCR. Bar graphs in (B), (C) and (E) represent mean ± SD ( n = 3).
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Gas5-derived small RNA (pi-sno75) specifically upregulates TRAIL expression in breast cancer cells. ( A ) The mRNA profiling <t>microarray</t> analysis. The gene expression scatterplot of the piRNAs mixture group versus that of the control group was shown. Significantly downregulated genes were shown in green, while upregulated genes in red. ( B ) MCF7 cells were transfected with piRNAs derived from GAS5 respectively for 48h, TRAIL mRNA levels were analysed by quantitative RT-PCR (qRT-PCR) and normalized to GAPDH levels. ( C ) MCF7 cells were transfected with pi-sno75 at different concentrations. The qRT-PCR was performed to analyse the effect of pi-sno75 on TRAIL expression. ( D ) MCF7 cells were transfected with pi-sno75 and piNC for 48h, western blot was performed to analyse TRAIL protein expression. ( E ) In MCF7 cells transfected with pi-sno75, the mRNA levels of TRAIL and other genes were determined by qRT-PCR. Bar graphs in (B), (C) and (E) represent mean ± SD ( n = 3).
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Gas5-derived small RNA (pi-sno75) specifically upregulates TRAIL expression in breast cancer cells. ( A ) The mRNA profiling <t>microarray</t> analysis. The gene expression scatterplot of the piRNAs mixture group versus that of the control group was shown. Significantly downregulated genes were shown in green, while upregulated genes in red. ( B ) MCF7 cells were transfected with piRNAs derived from GAS5 respectively for 48h, TRAIL mRNA levels were analysed by quantitative RT-PCR (qRT-PCR) and normalized to GAPDH levels. ( C ) MCF7 cells were transfected with pi-sno75 at different concentrations. The qRT-PCR was performed to analyse the effect of pi-sno75 on TRAIL expression. ( D ) MCF7 cells were transfected with pi-sno75 and piNC for 48h, western blot was performed to analyse TRAIL protein expression. ( E ) In MCF7 cells transfected with pi-sno75, the mRNA levels of TRAIL and other genes were determined by qRT-PCR. Bar graphs in (B), (C) and (E) represent mean ± SD ( n = 3).
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Gas5-derived small RNA (pi-sno75) specifically upregulates TRAIL expression in breast cancer cells. ( A ) The mRNA profiling <t>microarray</t> analysis. The gene expression scatterplot of the piRNAs mixture group versus that of the control group was shown. Significantly downregulated genes were shown in green, while upregulated genes in red. ( B ) MCF7 cells were transfected with piRNAs derived from GAS5 respectively for 48h, TRAIL mRNA levels were analysed by quantitative RT-PCR (qRT-PCR) and normalized to GAPDH levels. ( C ) MCF7 cells were transfected with pi-sno75 at different concentrations. The qRT-PCR was performed to analyse the effect of pi-sno75 on TRAIL expression. ( D ) MCF7 cells were transfected with pi-sno75 and piNC for 48h, western blot was performed to analyse TRAIL protein expression. ( E ) In MCF7 cells transfected with pi-sno75, the mRNA levels of TRAIL and other genes were determined by qRT-PCR. Bar graphs in (B), (C) and (E) represent mean ± SD ( n = 3).
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human genome u133 + 2.0 oligonucleotide arrays  (Thermo Fisher)
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Gas5-derived small RNA (pi-sno75) specifically upregulates TRAIL expression in breast cancer cells. ( A ) The mRNA profiling <t>microarray</t> analysis. The gene expression scatterplot of the piRNAs mixture group versus that of the control group was shown. Significantly downregulated genes were shown in green, while upregulated genes in red. ( B ) MCF7 cells were transfected with piRNAs derived from GAS5 respectively for 48h, TRAIL mRNA levels were analysed by quantitative RT-PCR (qRT-PCR) and normalized to GAPDH levels. ( C ) MCF7 cells were transfected with pi-sno75 at different concentrations. The qRT-PCR was performed to analyse the effect of pi-sno75 on TRAIL expression. ( D ) MCF7 cells were transfected with pi-sno75 and piNC for 48h, western blot was performed to analyse TRAIL protein expression. ( E ) In MCF7 cells transfected with pi-sno75, the mRNA levels of TRAIL and other genes were determined by qRT-PCR. Bar graphs in (B), (C) and (E) represent mean ± SD ( n = 3).
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Gas5-derived small RNA (pi-sno75) specifically upregulates TRAIL expression in breast cancer cells. ( A ) The mRNA profiling <t>microarray</t> analysis. The gene expression scatterplot of the piRNAs mixture group versus that of the control group was shown. Significantly downregulated genes were shown in green, while upregulated genes in red. ( B ) MCF7 cells were transfected with piRNAs derived from GAS5 respectively for 48h, TRAIL mRNA levels were analysed by quantitative RT-PCR (qRT-PCR) and normalized to GAPDH levels. ( C ) MCF7 cells were transfected with pi-sno75 at different concentrations. The qRT-PCR was performed to analyse the effect of pi-sno75 on TRAIL expression. ( D ) MCF7 cells were transfected with pi-sno75 and piNC for 48h, western blot was performed to analyse TRAIL protein expression. ( E ) In MCF7 cells transfected with pi-sno75, the mRNA levels of TRAIL and other genes were determined by qRT-PCR. Bar graphs in (B), (C) and (E) represent mean ± SD ( n = 3).
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Image Search Results


Gas5-derived small RNA (pi-sno75) specifically upregulates TRAIL expression in breast cancer cells. ( A ) The mRNA profiling microarray analysis. The gene expression scatterplot of the piRNAs mixture group versus that of the control group was shown. Significantly downregulated genes were shown in green, while upregulated genes in red. ( B ) MCF7 cells were transfected with piRNAs derived from GAS5 respectively for 48h, TRAIL mRNA levels were analysed by quantitative RT-PCR (qRT-PCR) and normalized to GAPDH levels. ( C ) MCF7 cells were transfected with pi-sno75 at different concentrations. The qRT-PCR was performed to analyse the effect of pi-sno75 on TRAIL expression. ( D ) MCF7 cells were transfected with pi-sno75 and piNC for 48h, western blot was performed to analyse TRAIL protein expression. ( E ) In MCF7 cells transfected with pi-sno75, the mRNA levels of TRAIL and other genes were determined by qRT-PCR. Bar graphs in (B), (C) and (E) represent mean ± SD ( n = 3).

Journal: Nucleic Acids Research

Article Title: An Lnc RNA (GAS5)/SnoRNA-derived piRNA induces activation of TRAIL gene by site-specifically recruiting MLL/COMPASS-like complexes

doi: 10.1093/nar/gkv214

Figure Lengend Snippet: Gas5-derived small RNA (pi-sno75) specifically upregulates TRAIL expression in breast cancer cells. ( A ) The mRNA profiling microarray analysis. The gene expression scatterplot of the piRNAs mixture group versus that of the control group was shown. Significantly downregulated genes were shown in green, while upregulated genes in red. ( B ) MCF7 cells were transfected with piRNAs derived from GAS5 respectively for 48h, TRAIL mRNA levels were analysed by quantitative RT-PCR (qRT-PCR) and normalized to GAPDH levels. ( C ) MCF7 cells were transfected with pi-sno75 at different concentrations. The qRT-PCR was performed to analyse the effect of pi-sno75 on TRAIL expression. ( D ) MCF7 cells were transfected with pi-sno75 and piNC for 48h, western blot was performed to analyse TRAIL protein expression. ( E ) In MCF7 cells transfected with pi-sno75, the mRNA levels of TRAIL and other genes were determined by qRT-PCR. Bar graphs in (B), (C) and (E) represent mean ± SD ( n = 3).

Article Snippet: Gene expression profiles were analysed with the Whole Human Genome Microarray (Human Genome U133 Plus 2.0, Affymetrix).

Techniques: Derivative Assay, Expressing, Microarray, Transfection, Quantitative RT-PCR, Western Blot